WebMar 13, 2024 · CL802 was used as a negative control for CL4176. (B) Thioflavin T staining assay. Worms prepared in the same manner as for the Aβ 42 paralysis assay were harvested three days after the growth temperature was increased to 25 °C. Worms stained with thioflavin T (green) and DAPI (nuclei; blue) were analyzed by confocal microscopy. WebApr 3, 2024 · Thioflavin T staining was used as a marker of ER stress and intracellular misfolded protein aggregation 32,33. The fraction of cells containing thioflavin puncta was quantified using the Image J ...
(PDF) Rational Generation of Monoclonal Antibodies Selective for ...
WebFeb 18, 2024 · The MCCP treatment did not affect the transcript abundance of amyloid-β transgene (amy-1); however, Thioflavin T staining showed a significant decrease in Aβ accumulation compared to untreated worms. Further research using transgenic worms found that MCCP promoted the translocation of DAF-16 from cytoplasm to nucleus and … WebMar 11, 2024 · It should be noted that thioflavin T can also be used for staining cultures, which shifts its excitation maximum from 385 to 450 nm only upon binding to β-pleated … austin22 xt2213-2
Thioflavine S practical grade 1326-12-1 - Sigma-Aldrich
WebAug 22, 2024 · Congo red and thioflavin-T stains are negative in the glomeruli, interstitium, and vasculatures. ... (20 nm diameter) that did not stain with thioflavin or Congo red . It was also differentiated ultrastructurally from immunotactoid glomerulopathy at a later date by the random orientation of the fibrils, ... WebJan 1, 2012 · Thioflavin stain is viewed under a fluorescein gate using a standard immunofluorescence microscope. Thioflavin T fluoresces only … WebDec 1, 2024 · Note that thioflavin T staining is a widely used method to follow amyloids self-assembly. Nevertheless in the case of Hfq-CTR, thioflavin inhibits fibril formation and cannot be used [ 41 ]. As a model we first used the 11 amino acid residues peptide corresponding to the Hfq amyloid region only, referred to as Hfq CTR 11 [ 42 ] (see sequence in ... austin 2370