Phosphospecific antibody
WebPhospho-Specific Antibodies. Protein Phosphorylation involves the addition of phosphate groups to proteins, most commonly serine (S), threonine (T) or tyrosine (Y) residue by a … WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 950 publications. Validated for WB, IP, IHC. Highly specific and rigorously validated in-house, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit Monoclonal Antibody (CST #4376) is ready to ship.
Phosphospecific antibody
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WebAntiphosphotyrosine antibodies have been proven very successful in purification, but fewer reports have been published using antibodies against phosphoserine- or phosphothreonine-containing proteins. IMAC enrichment is based on phosphate affinity for immobilized metal chelated to the resin. WebAsc (Tyr-144), phospho-specific Antibody Catalog # AP5631 Asc (Tyr-144), phospho-specific Antibody Rabbit Polyclonal Application / Dilution Size 100 μl Species Reactivity …
WebThe immunoprecipitation with the T172-phosphospecific mAbs followed by detection with a CDK4 antibody was more efficient than the converse combination. (c) Urea extracts of MCF7 or mouse C2C12 cells were separated by 2D-gel electrophoresis and detected by Western blotting either with total CDK4 DCS-31 antibody or with NB8-AD9 or LA2-AD4 phospho ... WebOct 10, 2013 · For the most part, phosphospecific antibodies can be treated just like any other antibody. But there are some differences. For instance, phosphates in the buffers can result in nonspecific signals, unlike with conventional antibodies. “The common blocking solution, 5% nonfat milk, contains phosphorylated proteins which may nonspecifically ...
WebFeb 25, 2016 · Using a new phosphospecific antibody directed to the phosphorylation consensus we found Protein Kinase A and Calcium/Calmodulin-dependent Protein Kinase II to robustly phosphorylate CPEB3 in vitro and in primary hippocampal neurons. WebCustom Monoclonal Antibodies; CellPower™ Assay Cell Line Development; DIAGNOSTICS Hot! COVID-19. cPass™ Technology . Rapid Tests. Resource . News . cPass Technology ... Using a new phosphospecific antibody (pPC2) to this site, we demonstrate that Ser(829) is phosphorylated by Protein kinase A (PKA) but remains constitutively phosphorylated ...
WebRabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1/ABL2. Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y393/Y429 of human ABL1/ABL2. (PAB12190) - Products - Abnova
WebAliquot an optimal concentration of phosphospecific fluorochrome-conjugated antibody to each tube. Incubate at room temperature for 30 minutes in the dark. Wash once with 2 mL of Stain Buffer. Centrifuge at 300g for 5–10 minutes and aspirate the supernatant. Resuspend in 500 μL of the same buffer prior to flow cytometric analysis. simpsons meme worst day so farWebPhosphoproteomics is a branch of proteomics that identifies, catalogs, and characterizes proteins containing a phosphate group as a posttranslational modification. … razor claw before elite fourWebPhosphospecific Antibody Finally, a phosphospecific antibody recognizing Ser304 on PDE4B was developed, and this revealed that incubation of mouse hepatocytes with either 991 (1–10μM) or phenformin (50–500μM) for 1h led to phosphorylation at that site. From: Reference Module in Biomedical Sciences, 2024 View all Topics Download as PDF razorclaw bearWebImmunogen. Synthetic peptide corresponding to human STAT4 phosphorylated at tyrosine 693. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. simpsons megaphone testingWebOct 8, 2013 · Antibodies specific to phosphorylated peptides are readily generated through structure-based engineering and in vitro selection. The study of post-translational … razor claw bearWebThe development of phospho-specific antibodies to detect and quantify protein phosphorylation made life easier for researchers (less 32P waste to deal with), but the … razor claw beast machinesWebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies provide critical insight into the investigation of complex signal transductions events. On this page … simpsons meme say the line